Thanks Maximise and Sven, both very informative answers.

I have changed the question to a discussion as it seems there may be no definitive answer  to the above, so look forward to hopefully seeing further input from the Moz community.

Link spam is naturally a hot topic amongst SEO's, particularly post Penguin. While digging around forums etc, I watched a video blog from Matt Cutts posted a while ago that suggests that Google only pays attention to the first instance of a link on the page

As most websites will have multiple instances of a links (header, footer and body text), is it beneficial to nofollow the additional instances of the link?

Also as the first instance of a link will in most cases be within the header nav, does that then make the content link text critical or can good on page optimisation be pulled from the title attribute?

I would appreciate the experiences and thoughts Mozzers thoughts on this

thanks in advance!

Added X robots tag into our headers on our development sites.

Just a note - if you use apache and have mod_pagespeed installed , it wall conflict and pagespeed will remove the X robots tag.

Begin Bad Bot Blocking

BrowserMatchNoCase Googlebot bad_bot
BrowserMatchNoCase bingbot bad_bot
BrowserMatchNoCase OmniExplorer_Bot/6.11.1 bad_bot
BrowserMatchNoCase omniexplorer_bot bad_bot
BrowserMatchNoCase Baiduspider bad_bot
BrowserMatchNoCase Baiduspider/2.0 bad_bot
BrowserMatchNoCase yandex bad_bot
BrowserMatchNoCase yandeximages bad_bot
BrowserMatchNoCase Spinn3r bad_bot
BrowserMatchNoCase sogou bad_bot
BrowserMatchNoCase Sogouwebspider/3.0 bad_bot
BrowserMatchNoCase Sogouwebspider/4.0 bad_bot
BrowserMatchNoCase sosospider+ bad_bot
BrowserMatchNoCase jikespider bad_bot
BrowserMatchNoCase ia_archiver bad_bot
BrowserMatchNoCase PaperLiBot bad_bot
BrowserMatchNoCase ahrefsbot bad_bot
BrowserMatchNoCase ahrefsbot/1.0 bad_bot
BrowserMatchNoCase SiteBot/0.1 bad_bot
BrowserMatchNoCase DNS-Digger/1.0 bad_bot
BrowserMatchNoCase DNS-Digger-Explorer/1.0 bad_bot
BrowserMatchNoCase boardreader bad_bot
BrowserMatchNoCase radian6 bad_bot
BrowserMatchNoCase R6_FeedFetcher bad_bot
BrowserMatchNoCase R6_CommentReader bad_bot
BrowserMatchNoCase ScoutJet bad_bot
BrowserMatchNoCase ezooms bad_bot
BrowserMatchNoCase CC-rget/5.818 bad_bot
BrowserMatchNoCase libwww-perl/5.813 bad_bot
BrowserMatchNoCase magpie-crawler 1.1 bad_bot
BrowserMatchNoCase jakarta bad_bot
BrowserMatchNoCase discobot/1.0 bad_bot
BrowserMatchNoCase MJ12bot bad_bot
BrowserMatchNoCase MJ12bot/v1.2.0 bad_bot
BrowserMatchNoCase MJ12bot/v1.2.5 bad_bot
BrowserMatchNoCase SemrushBot/0.9 bad_bot
BrowserMatchNoCase MLBot bad_bot
BrowserMatchNoCase butterfly bad_bot
BrowserMatchNoCase SeznamBot/3.0 bad_bot
BrowserMatchNoCase HuaweiSymantecSpider bad_bot
BrowserMatchNoCase Exabot/2.0 bad_bot
BrowserMatchNoCase netseer/0.1 bad_bot
BrowserMatchNoCase NetSeer crawler/2.0 bad_bot
BrowserMatchNoCase NetSeer/Nutch-0.9 bad_bot
BrowserMatchNoCase psbot/0.1 bad_bot
BrowserMatchNoCase Moreoverbot/x.00 bad_bot
BrowserMatchNoCase moreoverbot/5.0 bad_bot
BrowserMatchNoCase Jakarta Commons-HttpClient/3.0 bad_bot
BrowserMatchNoCase SocialSpider-Finder/0.2 bad_bot
BrowserMatchNoCase MaxPointCrawler/Nutch-1.1 bad_bot
BrowserMatchNoCase willow bad_bot
Order Deny,Allow
Deny from env=bad_bot

End Bad Bot Blocking

Header set X-Robots-Tag "noindex, nofollow"

Begin Bad Bot Blocking

BrowserMatchNoCase Googlebot bad_bot
BrowserMatchNoCase bingbot bad_bot
BrowserMatchNoCase OmniExplorer_Bot/6.11.1 bad_bot
BrowserMatchNoCase omniexplorer_bot bad_bot
BrowserMatchNoCase Baiduspider bad_bot
BrowserMatchNoCase Baiduspider/2.0 bad_bot
BrowserMatchNoCase yandex bad_bot
BrowserMatchNoCase yandeximages bad_bot
BrowserMatchNoCase Spinn3r bad_bot
BrowserMatchNoCase sogou bad_bot
BrowserMatchNoCase Sogouwebspider/3.0 bad_bot
BrowserMatchNoCase Sogouwebspider/4.0 bad_bot
BrowserMatchNoCase sosospider+ bad_bot
BrowserMatchNoCase jikespider bad_bot
BrowserMatchNoCase ia_archiver bad_bot
BrowserMatchNoCase PaperLiBot bad_bot
BrowserMatchNoCase ahrefsbot bad_bot
BrowserMatchNoCase ahrefsbot/1.0 bad_bot
BrowserMatchNoCase SiteBot/0.1 bad_bot
BrowserMatchNoCase DNS-Digger/1.0 bad_bot
BrowserMatchNoCase DNS-Digger-Explorer/1.0 bad_bot
BrowserMatchNoCase boardreader bad_bot
BrowserMatchNoCase radian6 bad_bot
BrowserMatchNoCase R6_FeedFetcher bad_bot
BrowserMatchNoCase R6_CommentReader bad_bot
BrowserMatchNoCase ScoutJet bad_bot
BrowserMatchNoCase ezooms bad_bot
BrowserMatchNoCase CC-rget/5.818 bad_bot
BrowserMatchNoCase libwww-perl/5.813 bad_bot
BrowserMatchNoCase magpie-crawler 1.1 bad_bot
BrowserMatchNoCase jakarta bad_bot
BrowserMatchNoCase discobot/1.0 bad_bot
BrowserMatchNoCase MJ12bot bad_bot
BrowserMatchNoCase MJ12bot/v1.2.0 bad_bot
BrowserMatchNoCase MJ12bot/v1.2.5 bad_bot
BrowserMatchNoCase SemrushBot/0.9 bad_bot
BrowserMatchNoCase MLBot bad_bot
BrowserMatchNoCase butterfly bad_bot
BrowserMatchNoCase SeznamBot/3.0 bad_bot
BrowserMatchNoCase HuaweiSymantecSpider bad_bot
BrowserMatchNoCase Exabot/2.0 bad_bot
BrowserMatchNoCase netseer/0.1 bad_bot
BrowserMatchNoCase NetSeer crawler/2.0 bad_bot
BrowserMatchNoCase NetSeer/Nutch-0.9 bad_bot
BrowserMatchNoCase psbot/0.1 bad_bot
BrowserMatchNoCase Moreoverbot/x.00 bad_bot
BrowserMatchNoCase moreoverbot/5.0 bad_bot
BrowserMatchNoCase Jakarta Commons-HttpClient/3.0 bad_bot
BrowserMatchNoCase SocialSpider-Finder/0.2 bad_bot
BrowserMatchNoCase MaxPointCrawler/Nutch-1.1 bad_bot
BrowserMatchNoCase willow bad_bot
Order Deny,Allow
Deny from env=bad_bot

End Bad Bot Blocking

Header set X-Robots-Tag "noindex, nofollow"

have you tried the on page report card?

Assuming you have a campaign set up in the SeoMOZ web app, load up your keywords then go to

Campaign -> On-page -> report card

select the keyword and enter the url and your will get the page graded

As part of the report it shows ranking factors such as broad keyword usage, appropriate keyword usage, plus a whole host of other ranking factors.